HIV-1-based Lentiviral Vectors for Neuronal Gene Transfer
Author Information
Author(s): Thais Federici, Robert Kutner, Xian-Yang Zhang, Hitoshi Kuroda, Noël Tordo, Nicholas M Boulis, Jakob Reiser
Primary Institution: Emory University
Hypothesis
Can HIV-1-based lentiviral vectors pseudotyped with lyssavirus glycoproteins effectively transduce motor neurons?
Conclusion
HIV-1-based lentiviral vectors pseudotyped with the Rabies PV glycoprotein are promising for targeting the central nervous system via peripheral injection.
Supporting Evidence
- Rabies PV-pseudotyped vectors transduced 20.76% of SH-SY5Y cells at a low MOI.
- In NSC-34 cells, Rabies PV pseudotypes transduced 49.9% of the cells.
- In mixed spinal cord cultures, Rabies PV pseudotypes transduced 70.35% of neurons.
Takeaway
Scientists are testing a new way to deliver genes to brain cells using special viruses that can travel along nerves, which might help treat diseases like ALS.
Methodology
The study involved in vitro tests on neuroblastoma cells and in vivo tests with rat sciatic nerve injections to evaluate the transduction efficiency of different pseudotyped lentiviral vectors.
Limitations
The study primarily focused on specific pseudotypes and may not generalize to all lentiviral vectors.
Participant Demographics
Adult male Sprague Dawley rats were used for in vivo experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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