Buffy Coat Specimens as DNA Source for Genetic Tests
Author Information
Author(s): Mychaleckyj Josyf C, Farber Emily A, Chmielewski Jessica, Artale Jamie, Light Laney S, Bowden Donald W, Hou Xuanlin, Marcovina Santica M
Primary Institution: University of Virginia
Hypothesis
Can buffy coat specimens stored for up to 9 years still provide viable DNA for genetic testing?
Conclusion
Buffy coat specimens can be stored for up to 9 years at -80°C and still yield high-quality DNA suitable for genetic analysis.
Supporting Evidence
- DNA was isolated from all 120 specimens with yields ranging from 1.1 to 312 ug.
- Only 3 out of 120 samples yielded less than 10 ug of DNA.
- DNA quality was assessed as very good based on gel electrophoresis and genotyping results.
Takeaway
Scientists found that blood samples stored in a special way can still be used to get good DNA even after a long time in the freezer.
Methodology
DNA was isolated from 120 buffy coat specimens using two QIAGEN automated protocols and tested for quality and yield.
Potential Biases
Potential bias in sample selection for GWA genotyping due to substitution of lower yield samples.
Limitations
The study may not generalize to all types of blood samples or storage conditions.
Participant Demographics
35% female, 64% white, 19% African American, mean age 63.5 years.
Statistical Information
P-Value
0.009
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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