Effects of Glucose and Amino Acids on p27 Expression in Breast Cancer Cells
Author Information
Author(s): Isao Eto
Primary Institution: University of Alabama at Birmingham
Hypothesis
Can glucose levels and certain amino acids regulate the expression of the p27 protein in breast cancer cells?
Conclusion
The study found that 4-hydroxytamoxifen up-regulated p27 expression, while deficiency of glucose or L-leucine also increased p27 levels.
Supporting Evidence
- 4-hydroxytamoxifen up-regulated p27 expression using pathway #1.
- Deficiency of D-(+)-glucose or L-leucine up-regulated p27 expression using pathway #2.
- Moderate increase in D-(+)-glucose down-regulated p27 expression.
Takeaway
This study shows that certain nutrients can change how a protein related to cancer works in breast cells, which might help us understand cancer better.
Methodology
The study used p27-luciferase reporter transfection assays and western immunoblot analyses on human MDA-MB-231 breast cancer cells.
Limitations
The study was conducted in vitro, which may not fully represent in vivo conditions.
Participant Demographics
Human MDA-MB-231 breast cancer cells were used.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website