Efficient Gene Transfer in Airway Epithelium Using Lentiviral Vectors
Author Information
Author(s): Copreni Elena, Palmieri Lucia, Castellani Stefano, Conese Massimo
Primary Institution: Institute for the Experimental Treatment of Cystic Fibrosis, H.S. Raffaele, Milan, Italy
Hypothesis
The study aims to evaluate the efficiency and duration of gene expression mediated by a VSV-G pseudotyped last generation lentiviral vector.
Conclusion
A last generation lentiviral vector can mediate efficient and sustained gene expression in the mouse airways.
Supporting Evidence
- Primary bronchial and nasal epithelial cells were transduced up to 70–80% 72 hours after lentiviral infection.
- In vivo luciferase expression lasted for at least 6 months without decline.
- Lysophosphatidylcholine pre-treatment enhanced nasal luciferase activity significantly.
Takeaway
Researchers found a new way to deliver genes into lung cells that works really well and lasts a long time, which could help treat diseases like cystic fibrosis.
Methodology
The study used ex-vivo models of respiratory epithelial cells and in vivo models in C57Bl/6 mice to evaluate gene transfer efficiency and persistence.
Limitations
The study did not examine the long-term expression of the bronchial epithelium beyond four weeks.
Participant Demographics
C57Bl/6 mice were used for in vivo experiments.
Statistical Information
P-Value
0.02
Statistical Significance
p=0.02
Digital Object Identifier (DOI)
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