Impact of a Mutation in the IL-6 Receptor on Arthritis in Mice
Author Information
Author(s): Tsuji Fumio, Yoshimi Miwa, Katsuta Osamu, Takai Miwa, Ishihara Katsuhiko, Aono Hiroyuki
Primary Institution: Santen Pharmaceutical Co., Ltd.
Hypothesis
The point mutation of tyrosine 759 in the IL-6 receptor gp130 affects the severity of collagen-induced arthritis in DBA/1J mice.
Conclusion
The Tyr-759 point mutation of the IL-6 family cytokine receptor subunit, gp130, caused autoimmune disease, influenced by genetic background.
Supporting Evidence
- Mice with the mutation showed more severe arthritis compared to controls.
- Methotrexate treatment reduced arthritis severity in the mutant mice.
- The study highlights the role of genetic background in disease manifestation.
Takeaway
A small change in a protein can make mice get arthritis more easily, and this can help scientists test new medicines faster.
Methodology
The study involved backcrossing gp130F759 mice to DBA/1J and C57BL/6 backgrounds, inducing collagen-induced arthritis, and evaluating the effects of methotrexate.
Potential Biases
Potential bias in the selection of genetic backgrounds and the interpretation of results.
Limitations
The study primarily focused on two genetic backgrounds and may not represent all variations of arthritis.
Participant Demographics
Mice used were of the DBA/1J and C57BL/6 genetic backgrounds.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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