COX Activity and Mouse Hepatitis Virus Replication
Author Information
Author(s): Raaben Matthijs, Einerhand Alexandra WC, Taminiau Lucas JA, van Houdt Michel, Bouma Janneke, Raatgeep Rolien H, Büller Hans A, de Haan Cornelis AM, Rossen John WA
Primary Institution: Utrecht University, Utrecht, The Netherlands
Hypothesis
COX activity is required for efficient replication of mouse hepatitis virus (MHV) at an early stage of infection.
Conclusion
COX activity is essential for MHV replication, suggesting it could be a target for anti-coronaviral therapy.
Supporting Evidence
- Blocking COX activity reduced MHV infection by 57% with indomethacin and 95% with curcumine.
- COX-2 activity was confirmed to be important for MHV replication through RNA interference.
- Inhibition of COX activity led to a significant decrease in the production of infectious viral progeny.
Takeaway
The study found that certain chemicals that block COX activity can stop a virus from making copies of itself, which could help in treating infections.
Methodology
Caco-2 cells were infected with MHV in the presence of COX inhibitors, and the number of infected cells was measured using immunofluorescence.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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