Effects of Ebp1 Deficiency on Mouse Growth and Signaling
Author Information
Author(s): Zhang Yuexing, Lu Yan, Zhou Hua, Lee Myounghee, Liu Zhenqiu, Hassel Bret A, Hamburger Anne W
Primary Institution: University of Maryland School of Medicine
Hypothesis
The physiological role of Ebp1 in the whole organism is not known.
Conclusion
Ebp1 deficiency leads to reduced growth and altered expression of proliferation-related genes in mice.
Supporting Evidence
- Ebp1-/- mice were on average 30% smaller than wild type and heterozygous littermates.
- Growth retardation was observed from Day 10 until Day 30.
- IGF-1 production was reduced in Ebp1-/- mice.
- Proliferation of fibroblasts from Ebp1-/- embryos was decreased compared to wild type.
Takeaway
Mice without the Ebp1 gene are smaller than normal mice, but they catch up in size as they grow older.
Methodology
Ebp1-deficient mice were generated using a gene trap insertion, and various assays were performed to assess growth and gene expression.
Limitations
The study does not explore the long-term effects of Ebp1 deficiency beyond one year.
Participant Demographics
Mice were of mixed genetic background (C57BL/6 and 129SvEvBrd).
Statistical Information
P-Value
p = .008
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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