Identifying Genes Affected by Adenovirus Transformation
Author Information
Author(s): Janet Strath, Lindsay J Georgopoulos, Paul Kellam, G Eric Blair
Primary Institution: University of Leeds
Hypothesis
The comparative analysis of gene profiles from untransformed BRK cells and BRK cells transformed with Ad12 or Ad5 E1 will reveal expression differences between cells transformed with oncogenic versus non-oncogenic adenovirus serotypes.
Conclusion
The study found that adenovirus transformation alters a complex web of cellular pathways, with specific genes being dysregulated in transformed cells.
Supporting Evidence
- 232 genes were differentially expressed in Ad12 E1- or Ad5 E1-transformed BRK cells compared to untransformed BRK cells.
- Real-time PCR and Western blotting confirmed the up-regulation of specific genes in transformed cells.
- Gene ontology analysis classified the majority of dysregulated genes into categories related to signal transduction and transcription.
Takeaway
The study looked at how certain viruses change the way cells work, finding that some genes behave differently when cells are transformed by these viruses.
Methodology
The study used microarray analysis to compare gene expression in transformed and untransformed BRK cells.
Limitations
The study is limited by the number of genes analyzed and the sensitivity of the microarray technique.
Participant Demographics
Primary BRK cells were isolated from 5 to 7 day old Wistar rats.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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