A Flexible Approach for Highly Multiplexed Candidate Gene Targeted Resequencing
Author Information
Author(s): Natsoulis Georges, Bell John M., Xu Hua, Buenrostro Jason D., Ordonez Heather, Grimes Susan, Newburger Daniel, Jensen Michael, Zahn Jacob M., Zhang Nancy, Ji Hanlee P.
Primary Institution: Stanford University School of Medicine
Hypothesis
We have developed an integrated strategy for targeted resequencing and analysis of gene subsets from the human exome for variants.
Conclusion
The study presents a cost-effective method for targeted resequencing of gene subsets that can be applied to various genetic studies.
Supporting Evidence
- The capture technology allows for resequencing of gene subsets larger than simplex PCR but smaller than exome sequencing.
- The Human OligoExome resource is publicly available for researchers to design custom capture assays.
- The method has been successfully applied to various genomic targets with high specificity.
Takeaway
The researchers created a new way to look at many genes at once to find changes that might cause diseases, using less DNA than usual.
Methodology
The study used in-solution capture with 80-mer oligonucleotides to target and resequence gene subsets from the human exome.
Limitations
The method may have reduced target coverage due to the presence of homologous genes.
Digital Object Identifier (DOI)
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