Gene Methylation Profiles in Colorectal Tumors
Author Information
Author(s): Ahlquist Terje, Lind Guro E, Costa Vera L, Meling Gunn I, Vatn Morten, Hoff Geir S, Rognum Torleiv O, Skotheim Rolf I, Thiis-Evensen Espen, Lothe Ragnhild A
Primary Institution: Norwegian Radium Hospital, Rikshospitalet University Hospital, Oslo, Norway
Hypothesis
This study aims to pinpoint epigenetic markers that can discriminate between non-malignant and malignant tissue from the large bowel.
Conclusion
Methylated ADAMTS1, MGMT, and MAL are suitable as markers for early tumor detection.
Supporting Evidence
- The mean number of methylated genes per sample was significantly different among normal mucosa, adenomas, and carcinomas.
- Hypermethylated CRABP1, MLH1, NR3C1, RUNX3, and SCGB3A1 were shown to be identifiers of carcinomas with microsatellite instability.
- Overall, 6/21 of the normal samples, 9/18 of the cancer patient samples, 52/63 of the adenomas, and 48/52 of the carcinomas were methylated in one or more of the analyzed genes.
Takeaway
The study found that certain genes are more likely to be methylated in cancerous tissues compared to normal tissues, which can help in early cancer detection.
Methodology
The methylation status of eleven genes was determined in 154 tissue samples including normal mucosa, adenomas, and carcinomas.
Participant Demographics
The study included samples from cancer-free individuals and colorectal cancer patients, with median ages ranging from 52.5 to 70.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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