The critical role of ERK in death resistance and invasiveness of hypoxia-selected glioblastoma cells
2009

The Role of ERK in Glioblastoma Cells' Resistance to Death and Invasiveness Under Hypoxia

Sample size: 20 publication 10 minutes Evidence: moderate

Author Information

Author(s): Kim Jee-Youn, Kim Yong-Jun, Lee Sun, Park Jae-Hoon

Primary Institution: Kyung Hee University

Hypothesis

Chronic hypoxia induces phenotypic changes in glioblastoma cells that enhance their death resistance and invasiveness.

Conclusion

The study suggests that ERK activation is crucial for the death resistance and invasive behavior of glioblastoma cells under chronic hypoxia.

Supporting Evidence

  • Chronic hypoxia led to a significant increase in the survival of glioblastoma cells.
  • Hypoxia-selected cells showed higher levels of anti-apoptotic proteins compared to parent cells.
  • ERK activation was found to be a key factor in the invasive potential of glioblastoma cells.
  • Immunohistochemical analysis revealed a correlation between p-ERK expression and tumor grade.

Takeaway

When brain cancer cells are exposed to low oxygen for a long time, they become better at surviving and spreading, and this is partly because of a protein called ERK.

Methodology

The study involved selecting hypoxia-resistant glioblastoma cells, assessing cell death rates, protein expression, and invasiveness through various assays.

Limitations

The study primarily focuses on one cell line and may not fully represent the behavior of all glioblastoma cells.

Participant Demographics

Twenty cases of astrocytic glial tumor samples were analyzed, including various grades of tumors.

Statistical Information

P-Value

0.006

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2407-9-27

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