A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
2008
A New System to Detect Protein Cleavage in Cells
publication
Evidence: high
Author Information
Author(s): Ketteler Robin, Sun Zairen, Kovacs Karl F, He Wei-Wu, Seed Brian
Primary Institution: Center for Computational and Integrative Biology, Massachusetts General Hospital
Hypothesis
Can a new luciferase-based system effectively detect intracellular proteolysis?
Conclusion
The study presents a novel assay that can monitor proteolytic cleavage in living cells using a Gaussia luciferase-based system.
Supporting Evidence
- The luciferase release assay can detect cleavage of both short peptides and full-length proteins.
- The assay is compatible with high-throughput screening methodologies.
- The system allows for non-invasive monitoring of protease activity in living cells.
Takeaway
Researchers created a new way to see if proteins inside cells are being cut up, which is important for understanding how cells work.
Methodology
The study developed a luciferase-based reporter system that detects proteolytic cleavage by fusing Gaussia luciferase to β-actin with protease cleavage sites in between.
Digital Object Identifier (DOI)
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