Isolation of a Human Anti-HIV Antibody from a Single B Cell
Author Information
Author(s): Morris Lynn, Chen Xi, Alam Munir, Tomaras Georgia, Zhang Ruijun, Marshall Dawn J., Chen Bing, Parks Robert, Foulger Andrew, Jaeger Frederick, Donathan Michele, Bilska Mira, Gray Elin S., Abdool Karim Salim S., Kepler Thomas B., Whitesides John, Montefiori David, Moody M. Anthony, Liao Hua-Xin, Haynes Barton F.
Primary Institution: Duke Human Vaccine Institute and Departments of Medicine, Surgery and Immunology, Duke University School of Medicine, Durham, North Carolina, United States of America
Hypothesis
Can we isolate a human neutralizing monoclonal antibody against HIV-1 from a single memory B cell?
Conclusion
The study successfully isolated a novel anti-HIV-1 monoclonal antibody, CAP206-CH12, which neutralizes a subset of HIV-1 viruses.
Supporting Evidence
- The isolated antibody, CAP206-CH12, neutralized a subset of HIV-1 pseudoviruses.
- CAP206-CH12 utilized the same germ-line variable heavy and light chain gene families as the broadly neutralizing antibody 4E10.
- The study highlights the potential for isolating additional neutralizing antibodies from HIV-infected individuals.
Takeaway
Scientists found a special antibody in a person infected with HIV that can help fight the virus. This could help make better vaccines.
Methodology
The researchers used single cell sorting of memory B cells and PCR amplification to isolate the antibody.
Limitations
The isolated antibody did not neutralize all viruses sensitive to the individual's plasma, indicating that additional antibodies may be needed for full coverage.
Participant Demographics
The study involved a single HIV-1 subtype C chronically infected individual from South Africa.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website