Transcriptome Analysis of Diamondback Moth Larvae Parasitized by a Wasp
Author Information
Author(s): Etebari Kayvan, Palfreyman Robin W, Schlipalius David, Nielsen Lars K, Glatz Richard V, Asgari Sassan
Primary Institution: The University of Queensland
Hypothesis
How does parasitization by Diadegma semiclausum affect the transcriptome of Plutella xylostella larvae?
Conclusion
The study reveals significant changes in the expression of immune-related genes in P. xylostella larvae following parasitization by D. semiclausum.
Supporting Evidence
- 928 insect host transcripts showed significant expression changes after parasitization.
- Antimicrobial peptides like gloverin and cecropin E were up-regulated in parasitized larvae.
- 19 unique sequences from five PDV gene families were identified in the host.
Takeaway
When a wasp lays eggs in diamondback moth larvae, it changes how the larvae's genes work, especially those that help fight off infections.
Methodology
The study used deep sequencing to analyze the transcriptome of P. xylostella larvae after parasitization by D. semiclausum.
Potential Biases
Pooling RNA from different time points may mask individual gene expression changes over time.
Limitations
The pooled RNA samples may lead to bias in expression data as temporal expression information was lost.
Participant Demographics
The study focused on 3rd and 4th instar larvae of Plutella xylostella.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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