STAT3 Knockdown Reduces Pancreatic Cancer Cell Invasiveness
Author Information
Author(s): Li Hai dong, Huang Chen, Huang Ke jian, Wu Wei dong, Jiang Tao, Cao Jun, Feng Zhen zhong, Qiu Zheng jun
Primary Institution: Affiliated First People's Hospital, Shanghai Jiao Tong University, Shanghai, China
Hypothesis
The study investigates the effects of STAT3 knockdown on pancreatic cancer cell invasiveness and gene expression in nude mice.
Conclusion
STAT3 plays a crucial role in regulating tumor growth, invasion, and angiogenesis in pancreatic cancer cells.
Supporting Evidence
- STAT3 shRNA successfully silenced STAT3 mRNA and protein expression in SW1990 cells.
- Growth rate of STAT3-silenced tumors was significantly reduced compared to control tumors.
- Microvessel density was significantly lower in STAT3-silenced tumors than in parental or control tumors.
Takeaway
Scientists found that turning off a gene called STAT3 made pancreatic cancer cells grow slower and invade less in mice.
Methodology
The study used a STAT3 shRNA lentiviral vector to silence STAT3 in SW1990 pancreatic cancer cells, followed by xenograft assays in nude mice to assess tumor growth and invasiveness.
Limitations
The study did not explore the long-term effects of STAT3 knockdown on metastasis in other organs.
Participant Demographics
Nude male BALB/c mice were used for the experiments.
Statistical Information
P-Value
0.004
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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