Calcium-binding S100 Protein Expression in Pterygium
Author Information
Author(s): Riau Andri K., Wong Tina T., Beuerman Roger W., Tong Louis
Primary Institution: Singapore Eye Research Institute
Hypothesis
The study aimed to investigate the presence of various S100 proteins in pterygium compared to normal conjunctiva.
Conclusion
Higher levels of S100A6, S100A8, and S100A9 expressions were detected in the pterygium tissue relative to normal conjunctiva.
Supporting Evidence
- S100A6 expression was strong in the superficial layer of pterygium epithelium but relatively weaker in normal conjunctiva.
- S100A8 and S100A9 were localized in the superficial layer of both pterygium and normal conjunctiva epithelium, with higher levels in pterygium.
- S100A11 was expressed in the basal cells of conjunctival epithelium but in the suprabasal layers of pterygium epithelium.
Takeaway
The study found that certain proteins are more active in pterygium, which is a growth on the eye, compared to normal eye tissue.
Methodology
Immunofluorescent staining, western blot, and quantitative real-time polymerase chain reaction (RT-PCR) were used to investigate protein expression.
Limitations
The study had a small patient sample and the uninvolved conjunctiva may not be totally normal.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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