Studying Energy Production in Zebrafish Embryos
Author Information
Author(s): Krista D. Stackley, Craig C. Beeson, Jennifer J. Rahn, Sherine S. L. Chan, Alicia J. Kowaltowski
Primary Institution: Medical University of South Carolina
Hypothesis
Can we screen bioenergetics and mitochondrial function in the developing zebrafish embryo?
Conclusion
The study developed a method to measure mitochondrial function in zebrafish embryos, showing that respiration and acid extrusion increase with development.
Supporting Evidence
- Total basal respiration increased linearly from 3 to 48 hpf.
- Acid extrusion by zebrafish embryos was not primarily due to lactic acid production.
- Proton leak was highest during the segmentation period of embryonic development.
Takeaway
Scientists figured out how to measure energy production in baby fish called zebrafish, which helps us understand how their bodies work as they grow.
Methodology
The study used the XF24 extracellular flux analyzer to measure respiration and acid extrusion in zebrafish embryos at different developmental stages.
Potential Biases
Potential bias due to the use of embryos from different breeding pairs.
Limitations
The method may not account for all off-target effects of pharmacological agents used.
Participant Demographics
Zebrafish embryos at various developmental stages (3 hpf to 48 hpf).
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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