How Mesenchymal Stem Cells Move Across Blood Vessel Walls
Author Information
Author(s): Chamberlain Giselle, Smith Helen, Rainger G. Ed, Middleton Jim
Primary Institution: Leopold Muller Arthritis Research Centre, Medical School, Keele University, RJAH Orthopaedic Hospital, Oswestry, Shropshire, United Kingdom
Hypothesis
This study investigates how mesenchymal stem cells (MSCs) migrate across aortic endothelial cells and the effects of chemokines and shear stress on this process.
Conclusion
The study found that MSCs can adhere, crawl, and spread on endothelial cells, with these behaviors enhanced by the chemokine CXCL9 and shear stress.
Supporting Evidence
- MSCs showed increased adhesion and crawling on endothelial cells when the flow was stopped and then restarted.
- CXCL9 significantly enhanced the percentage of MSCs adhering, crawling, and spreading.
- MSCs migrated across endothelial cells in response to chemokines, with significant increases in migration observed.
Takeaway
This research shows that special cells called mesenchymal stem cells can move through blood vessel walls better when they are helped by certain signals and when the blood flow is just right.
Methodology
The study used flow assays and transwell migration assays to observe the behavior of murine MSCs in relation to murine aortic endothelial cells under various conditions.
Limitations
The study primarily used murine models, which may not fully represent human responses.
Participant Demographics
Murine mesenchymal stem cells were used, isolated from BALB/c mice aged 6-10 weeks.
Statistical Information
P-Value
p=0.04
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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