VEGF-A and CCL2 Levels in Indian ALS Patients
Author Information
Author(s): Pawan K Gupta, Sudesh Prabhakar, Chandrika Abburi, Neel K Sharma, Akshay Anand
Primary Institution: Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India
Hypothesis
The study investigates the mRNA levels of VEGF-A and CCL2 in Indian ALS patients to understand their association with extended survival.
Conclusion
The study found that VEGF-A and CCL2 mRNA levels are significantly elevated in the peripheral blood mononuclear cells of Indian ALS patients.
Supporting Evidence
- VEGF-A mRNA levels were found to be significantly elevated in ALS patients compared to controls.
- CCL2 mRNA levels were found to be unaltered when comparing different ALS classifications.
- Patients with respiratory dysfunction showed higher levels of VEGF-A and CCL2.
Takeaway
This study shows that certain proteins in the blood of ALS patients in India are higher than in healthy people, which might help them live longer.
Methodology
mRNA levels of VEGF-A and CCL2 were measured in PBMCs using Real Time PCR and compared with normal controls.
Potential Biases
Potential bias due to the convenience sampling of patients from a single center.
Limitations
The study is limited by its small sample size and the convenience sampling method.
Participant Demographics
Participants included 50 sporadic Indian ALS patients, with 25 definite, 15 probable, and 10 possible ALS cases.
Statistical Information
P-Value
p = 0.0001 for VEGF-A, p = 0.005 for CCL2
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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