CDC2 and SPDY in Oocyte Maturation
Author Information
Author(s): Hölzenspies Jurriaan J, Stoorvogel Willem, Colenbrander Ben, Roelen Bernard AJ, Gutknecht Dagmar R, van Haeften Theo
Primary Institution: Utrecht University
Hypothesis
The study investigates the role of CDC2 and its regulatory proteins in the localization and function during pig oocyte maturation.
Conclusion
CDC2 transiently associates with endoplasmic reticulum exit sites during oocyte maturation, suggesting a role in regulating the secretory pathway.
Supporting Evidence
- CDC2 was found to localize to a cluster of endoplasmic reticulum exit sites in porcine oocytes.
- Forskolin treatment inhibited the translocation of CDC2 to ERES, indicating its role in meiosis resumption.
- SPDY was shown to colocalize with CDC2 at ERES, suggesting a regulatory role during oocyte maturation.
Takeaway
This study shows that a protein called CDC2 helps oocytes get ready for fertilization by moving to special areas in the cell just before they mature.
Methodology
The study used immunofluorescence and confocal microscopy to analyze the localization of CDC2 and its regulatory proteins in porcine oocytes during maturation.
Limitations
The study primarily focuses on porcine oocytes, which may limit the generalizability of the findings to other species.
Participant Demographics
Porcine oocytes collected from sow ovaries.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website