miR-18a Impairs DNA Damage Response in Breast Cancer
Author Information
Author(s): Song Libing, Lin Chuyong, Wu Zhiqiang, Gong Hui, Zeng Yong, Wu Jueheng, Li Mengfeng, Li Jun
Primary Institution: Sun Yat-sen University, Guangzhou, Guangdong, China
Hypothesis
miR-18a downregulates ATM expression and affects DNA damage response in breast cancer cells.
Conclusion
The study found that miR-18a reduces the DNA damage repair ability and increases radiosensitivity in breast cancer cells by downregulating ATM expression.
Supporting Evidence
- miR-18a was significantly overexpressed in breast cancer cell lines and tissues compared to normal cells.
- Overexpression of miR-18a reduced ATM expression and impaired DNA damage repair.
- Inhibition of miR-18a increased ATM expression and reduced sensitivity to radiation.
Takeaway
This study shows that a tiny molecule called miR-18a can make breast cancer cells less able to fix their damaged DNA, which might help doctors find new ways to treat cancer.
Methodology
The study used real-time PCR, western blotting, flow cytometry, and clonogenic assays to analyze the effects of miR-18a on ATM expression and DNA damage response.
Limitations
The study primarily focused on breast cancer cell lines and may not fully represent the complexity of in vivo conditions.
Participant Demographics
The study included 19 breast cancer samples from patients diagnosed at Sun Yat-sen University.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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