CRABP1 and Its Role in Neuroblastoma Cells and Alzheimer's Disease
Author Information
Author(s): Uhrig Markus, Brechlin Peter, Jahn Olaf, Knyazev Yuri, Weninger Annette, Busia Laura, Honarnejad Kamran, Otto Markus, Hartmann Tobias
Primary Institution: Center for Molecular Biology of the University of Heidelberg (ZMBH)
Hypothesis
Does the upregulation of CRABP1 in neuroblastoma cells overproducing Aβ42 affect their differentiation potential?
Conclusion
Increasing the Aβ42/Aβ40 ratio up-regulates CRABP1, which reduces the differentiation potential of human neuroblastoma cells.
Supporting Evidence
- CRABP1 was the second most up-regulated protein in the study.
- An increased Aβ42/Aβ40 ratio led to a significant up-regulation of CRABP1.
- Knockdown of CRABP1 rescued the differentiation potential of neuroblastoma cells.
Takeaway
When certain brain cells make too much of a protein linked to Alzheimer's, they have a harder time growing and changing into other types of cells. But if we reduce that protein, they can grow better.
Methodology
A combined transcriptomics/proteomics analysis was performed to measure the effects of Aβ peptides in human neuroblastoma cells, validated by real-time PCR and RNA interference.
Limitations
The study does not distinguish between pure Aβ42 and Aβ40 effects due to the nature of intracellular processing.
Statistical Information
P-Value
0.0002
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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