Complete Genome Sequence of Bovine Herpesvirus 4 V.test Strain
Author Information
Author(s): Palmeira Leonor, Machiels Bénédicte, Lété Céline, Vanderplasschen Alain, Gillet Laurent
Primary Institution: University of Liège
Hypothesis
The study aims to determine the complete genome sequence of the BoHV-4 V.test strain and analyze its differences compared to other strains.
Conclusion
The complete genome sequence of the BAC-cloned BoHV-4 V.test strain was determined, revealing important genome organization features that could be significant for other herpesviruses.
Supporting Evidence
- The long unique coding region consists of 108,241 bp encoding at least 79 open reading frames.
- The study identified significant differences in genome organization compared to the BoHV-4 66-p-347 strain.
- The sequencing strategy used resulted in a high coverage of the viral genome.
Takeaway
Scientists figured out the full DNA sequence of a virus that affects cows, which helps us understand how similar viruses might work in humans.
Methodology
The complete sequence was determined using a pyrosequencing approach and BAC cloning.
Limitations
The study primarily focuses on the genomic sequence and does not address the functional implications of the identified features.
Digital Object Identifier (DOI)
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