Markers for Cancer Stem Cells in Lung Cancer
Author Information
Author(s): Nomura Masaharu, Fukuda Tetsuya, Fujii Kiyonaga, Kawamura Takeshi, Tojo Hiromasa, Kihara Makoto, Bando Yasuhiko, Gazdar Adi F, Tsuboi Masahiro, Oshiro Hisashi, Nagao Toshitaka, Ohira Tatsuo, Ikeda Norihiko, Gotoh Noriko, Kato Harubumi, Marko-Varga Gyorgy, Nishimura Toshihide
Primary Institution: Tokyo Medical University
Hypothesis
Can proteomic analysis of FFPE tissues identify specific protein markers for large cell neuroendocrine carcinoma (LCNEC) that distinguish it from small cell lung carcinoma (SCLC)?
Conclusion
The study identified four protein candidates that are preferentially expressed in LCNEC compared to SCLC, suggesting their potential as biomarkers.
Supporting Evidence
- Four proteins were identified as potential biomarkers for LCNEC.
- The study used laser microdissection to collect cancer cells from FFPE tissues.
- Statistical analysis confirmed the significance of the identified proteins.
- Immunohistochemistry supported the proteomic findings.
Takeaway
Researchers found special proteins in lung cancer that can help tell one type of cancer apart from another, which is important for better treatment.
Methodology
Proteins were extracted from laser microdissected cancer cells in FFPE tissues and analyzed using mass spectrometry.
Potential Biases
Potential bias in patient selection and the limited number of cases analyzed.
Limitations
The study had a small sample size and focused only on specific cancer types.
Participant Demographics
Patients included 4 with LCNEC, 5 with SCLC, and 5 with LCC, with a majority being male and ages ranging from 52 to 77.
Statistical Information
P-Value
6.1 × 10-4, 9.6 × 10-10, 3.9 × 10-10, 0.021
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website