Effects of Valproic Acid on Human Pericytes
Author Information
Author(s): Karén Jakob, Rodriguez Alejandro, Friman Tomas, Dencker Lennart, Sundberg Christian, Scholz Birger
Primary Institution: Uppsala University, Uppsala, Sweden
Hypothesis
The study investigates the effects of the histone deacetylase inhibitor valproic acid on pericyte proliferation, cell viability, migration, and differentiation.
Conclusion
Valproic acid inhibits pericyte proliferation and migration while promoting a phenotype associated with vessel stabilization.
Supporting Evidence
- Valproic acid exposure reduced pericyte proliferation by approximately 70%.
- HDAC inhibition through valproic acid did not significantly affect cell viability.
- Pericyte migration was significantly inhibited at higher concentrations of valproic acid.
- Valproic acid exposure altered the expression of angiogenesis-related genes in pericytes.
Takeaway
This study shows that a drug called valproic acid can stop certain cells from growing and moving, which might help in healing blood vessels.
Methodology
The study used in vitro experiments to assess the effects of valproic acid on human pericytes isolated from placentas, measuring proliferation, migration, and differentiation.
Limitations
The study is limited to in vitro conditions and may not fully represent in vivo responses.
Participant Demographics
Human pericytes isolated from full-term placentas.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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