HIV-1 Latency and Subtype Differences
Author Information
Author(s): Renée M van der Sluis, Georgios Pollakis, Marja L van Gerven, Ben Berkhout, Rienk E Jeeninga
Primary Institution: Academic Medical Centre, University of Amsterdam
Hypothesis
How do different HIV-1 subtypes affect proviral latency and transcriptional activation?
Conclusion
Subtype AE shows increased basal transcription levels but a reduced response to TNFα activation compared to other subtypes.
Supporting Evidence
- The majority of HIV-1 infected T cells only start viral gene expression after TNFα activation.
- Subtype AE combines increased basal transcription with a reduced TNFα response.
- TNFα treatment induced a significant 3-fold increase in CA-p24 positive cells.
Takeaway
HIV-1 can hide in the body and not show symptoms, but some types are better at hiding than others. This study looks at how different types of HIV-1 behave when trying to wake up from hiding.
Methodology
The study used a single round infection assay to measure HIV-1 transcriptional latency in T cell lines.
Limitations
The study primarily focused on subtype B and may not fully represent all HIV-1 subtypes.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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