Differential toll-like receptor 3 (TLR3) expression and apoptotic response to TLR3 agonist in human neuroblastoma cells
2011

TLR3 Expression and Response in Neuroblastoma Cells

Sample size: 14 publication Evidence: moderate

Author Information

Author(s): Chuang Jiin-Haur, Chuang Hui-Ching, Huang Chao-Cheng, Wu Chia-Ling, Du Yung-Ying, Kung Mei-Lang, Chen Chih-Hao, Chen San-Cher, Tai Ming-Hong

Primary Institution: Chang Gung Memorial Hospital-Kaohsiung Medical Center

Hypothesis

TLR3 may serve as a therapeutic target in human neuroblastoma.

Conclusion

The study shows that TLR3 is expressed in certain neuroblastoma cells and that its activation can induce apoptosis in high TLR3-expressing cells.

Supporting Evidence

  • TLR3 was significantly up-regulated in SK-N-AS cells compared to other cell lines.
  • Poly(I:C) treatment led to significant growth inhibition and apoptosis in high TLR3-expressing SK-N-AS cells.
  • Blockade of TLR3 signaling reduced the poly(I:C)-induced inhibition of cell proliferation and apoptosis.

Takeaway

Some neuroblastoma cells have a special protein called TLR3 that can help them die when treated with a specific medicine.

Methodology

The study used immunohistochemical analysis, quantitative RT-PCR, and various assays to evaluate TLR3 expression and its effects on neuroblastoma cells.

Limitations

The sample size was relatively small, which may limit the generalizability of the findings.

Participant Demographics

The study involved archival neuroblastic tumor specimens from 14 patients, including cases of neuroblastoma and ganglioneuroblastoma.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1423-0127-18-65

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication