Porcine adenovirus type 3 E1Blarge protein downregulates the induction of IL-8
2007
Porcine Adenovirus Type 3 E1Blarge Protein and IL-8 Production
publication
Evidence: moderate
Author Information
Author(s): Zhou Yan, Ficzycz Andrew, Tikoo Suresh Kumar
Primary Institution: Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Hypothesis
E1Blarge protein from porcine adenovirus type 3 inhibits the induction of IL-8 by affecting NF-κB transcription.
Conclusion
The study found that the E1Blarge protein downregulates IL-8 production by inhibiting NF-κB dependent transcription.
Supporting Evidence
- E1Blarge protein inhibits the transcription of IL-8 by affecting NF-κB.
- Immunofluorescence assays showed that E1Blarge prevents NF-κB from moving into the nucleus.
- Luciferase assays indicated reduced activity of the IL-8 promoter when E1Blarge was present.
Takeaway
The E1Blarge protein from a virus can stop the body from making a chemical that causes inflammation, which is important for fighting infections.
Methodology
HeLa cells were infected with various PAdV-3 mutants, and assays were performed to analyze IL-8 production and NF-κB activity.
Digital Object Identifier (DOI)
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