Protein Changes During Metamorphosis of Capitella sp. I
Author Information
Author(s): Chandramouli Kondethimmanahalli H, Soo Lisa, Qian Pei-Yuan
Primary Institution: KAUST Global Collaborative Research, Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, China
Hypothesis
The protein expression pattern during larval settlement and metamorphosis in the polychaete Capitella sp. I differs from that in the polycheates Pseudopolydora vexillosa and Hydroides elegans.
Conclusion
Differentially expressed proteins are likely involved in regulating the larval metamorphosis process and can serve as protein markers for studying molecular mechanisms associated with this process.
Supporting Evidence
- Twenty-three differentially expressed proteins were identified in the two developmental stages.
- Proteins related to cell division, cell migration, energy storage, and oxidative stress were plentifully expressed in competent larvae.
- Proteins involved in oxidative metabolism and transcriptional regulation were abundantly expressed in juveniles.
Takeaway
This study looked at how proteins change when the Capitella sp. I larvae turn into juveniles, helping us understand how they grow up.
Methodology
Two-dimensional gel electrophoresis (2-DE) followed by multiplex fluorescent staining and MALDI-TOF mass spectrometry analysis were used to identify differentially expressed proteins.
Limitations
The study did not identify all differentially expressed phosphoproteins due to difficulties in obtaining satisfactory protein identification.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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