Proteomic Analysis of Human Skin Treated with Larval Schistosome Peptidases Reveals Distinct Invasion Strategies among Species of Blood Flukes
2011

How Schistosome Parasites Invade Human Skin

publication 10 minutes Evidence: moderate

Author Information

Author(s): Ingram Jessica, Knudsen Giselle, Lim K. C., Hansell Elizabeth, Sakanari Judy, McKerrow James

Primary Institution: University of California San Francisco

Hypothesis

The primary invasive peptidase differs between schistosome species, with S. mansoni utilizing cercarial elastase and S. japonicum utilizing cathepsin B2.

Conclusion

The study shows that both S. mansoni cercarial elastase and cathepsin B2 can degrade skin proteins, but cathepsin B2 may be more effective due to its broader range of substrates.

Supporting Evidence

  • Both S. mansoni cercarial elastase and cathepsin B2 can cleave skin proteins.
  • Cathepsin B2 cleaves a broader range of substrates compared to cercarial elastase.
  • Different schistosome species have evolved distinct peptidases for skin invasion.

Takeaway

Schistosome parasites have special proteins that help them get into human skin. Different types of these parasites use different proteins to do this.

Methodology

Comparative proteomic analysis of human skin treated with purified cercarial elastase and cathepsin B2 to identify substrates.

Limitations

The study used a model peptidase from S. mansoni for S. japonicum due to the unavailability of active recombinant SjCB2.

Digital Object Identifier (DOI)

10.1371/journal.pntd.0001337

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