Assembly of Meiotic Centromeres in Male Mice
Author Information
Author(s): Parra María Teresa, Gómez Rocío, Viera Alberto, Llano Elena, Pendás Alberto M., Rufas Julio S., Suja José A.
Primary Institution: Universidad Autónoma de Madrid
Hypothesis
How do centromeric proteins assemble during male mouse meiosis?
Conclusion
The study reveals a sequential loading of centromeric proteins during male mouse meiosis, highlighting the importance of the interkinesis stage for centromere maturation.
Supporting Evidence
- Centromeric proteins Borealin, INCENP, and Aurora-B load sequentially during prophase I.
- Shugoshin 2 is necessary for the loading of MCAK at the inner centromere.
- The loading sequence of proteins is similar during prophase I and interkinesis.
- Outer kinetochore proteins BubR1 and CENP-E are the last to be assembled.
- Interkinesis is essential for centromere and kinetochore maturation.
Takeaway
This study shows how proteins that help chromosomes move during cell division are put together in a specific order when male mice make sperm.
Methodology
The researchers used immunofluorescence microscopy to analyze the loading sequence of centromeric proteins in mouse spermatocytes.
Participant Demographics
Adult male mice (C57BL/6 and Sgol2−/−)
Digital Object Identifier (DOI)
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