Dendritic Cell Activation by Photodynamic Treatment in Glioblastoma
Author Information
Author(s): Etminan N, Peters C, Lakbir D, Bünemann E, Börger V, Sabel M C, Hänggi D, Steiger H-J, Stummer W, Sorg R V
Primary Institution: Heinrich-Heine-University Hospital
Hypothesis
ALA/PDT of glioma promotes activation of dendritic cells.
Conclusion
ALA/PDT treatment of glioma spheroids promotes the initial steps of adaptive immunity, mediated by HSP-70.
Supporting Evidence
- ALA/PDT-treated spheroids attracted dendritic cells effectively.
- Dendritic cells matured after co-culture with ALA/PDT-treated spheroids.
- Blocking HSP-70 inhibited antigen uptake and dendritic cell maturation.
Takeaway
This study shows that a treatment called ALA/PDT helps special immune cells called dendritic cells to find and fight brain tumors.
Methodology
Human glioblastoma spheroids were treated with ALA/PDT, and the effects on dendritic cell attraction, antigen uptake, and maturation were studied in vitro.
Limitations
The study used established cell lines, which may not fully represent primary tumor cells in vivo.
Statistical Information
P-Value
0.0079, 0.0337, 0.0217, 0.0114, 0.0223, 0.0034, 0.0014, <0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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