Mapping of ESE-1 subdomains required to initiate mammary epithelial cell transformation via a cytoplasmic mechanism
2011
Mapping ESE-1 Subdomains for Mammary Cell Transformation
publication
Evidence: high
Author Information
Author(s): Jason D Prescott, Joanna M Poczobutt, John J Tentler, Darius M Walker, Arthur Gutierrez-Hartmann
Primary Institution: University of Colorado Denver
Hypothesis
ESE-1 initiates transformation of mammary epithelial cells via a cytoplasmic mechanism.
Conclusion
ESE-1 contains nuclear localization and export signals that are crucial for its function in mammary epithelial cell transformation.
Supporting Evidence
- ESE-1 is overexpressed in human breast cancer.
- ESE-1's SAR domain is necessary for its transforming function.
- Nuclear export signals in ESE-1 are critical for its activity.
Takeaway
ESE-1 is a protein that helps turn normal mammary cells into cancer cells by working in the cell's cytoplasm instead of the nucleus.
Methodology
The study used GFP fusion constructs to map nuclear localization and export signals in ESE-1 and assessed their roles in cell transformation.
Digital Object Identifier (DOI)
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