Tri6 Is a Global Transcription Regulator in the Phytopathogen Fusarium graminearum
Author Information
Author(s): Nasmith Charles G., Walkowiak Sean, Wang Li, Leung Winnie W. Y., Gong Yunchen, Johnston Anne, Harris Linda J., Guttman David S., Subramaniam Rajagopal
Primary Institution: Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada
Hypothesis
Tri6 is a global transcriptional regulator whose influence extends beyond the trichothecene gene cluster.
Conclusion
The study identifies Tri6 as a global regulator that can bind to and regulate genes involved in various aspects of cellular metabolism.
Supporting Evidence
- Tri6 regulates genes involved in the biosynthesis of the secondary metabolite deoxynivalenol (DON).
- ChIP-Seq revealed 198 additional targets potentially regulated by Tri6.
- Functional classification showed that ∼40% of the target genes are associated with cellular metabolism and transport.
- Expression profiling indicated that 49 out of 198 target genes are differentially regulated by Tri6 under nitrogen-limiting conditions.
Takeaway
Tri6 is like a boss that tells many different workers in a fungus how to do their jobs, especially when it comes to making a harmful chemical called DON.
Methodology
The study used chromatin immunoprecipitation followed by Illumina sequencing (ChIP-Seq) to identify gene targets of Tri6.
Digital Object Identifier (DOI)
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