Mutations Increase Virus Capsid Stability at High Temperatures
Author Information
Author(s): Lee Kuo Hao, Miller Craig R., Nagel Anna C., Wichman Holly A., Joyce Paul, Ytreberg F. Marty
Primary Institution: University of Idaho
Hypothesis
Do beneficial mutations that arise in a microbial population exposed to elevated temperature have stabilizing effects?
Conclusion
Most mutations observed in the study are stabilizing, enhancing the stability of the virus capsid at elevated temperatures.
Supporting Evidence
- Most mutations map to interfaces between viral coat proteins, suggesting they affect protein-protein interactions.
- Eight of the ten mutations have significantly negative binding affinities relative to the wildtype.
- Four of the ten mutations have decay rates significantly below the wildtype.
Takeaway
The study found that when viruses are exposed to higher temperatures, they can develop mutations that make them stronger and more stable.
Methodology
The study used molecular dynamics simulations and experimental decay assays to assess the stability of viral mutants.
Limitations
The study focused on a limited number of mutations and may not represent all possible mutations affecting stability.
Statistical Information
P-Value
p<0.05
Confidence Interval
(95% CI)
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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