Effects of Dimetylthiourea and N-Acetylcysteine on Nanoparticle Damage in Lung Cancer Cells
Author Information
Author(s): Srivastava Ritesh Kumar, Rahman Qamar, Kashyap Mahendra Pratap, Lohani Mohtashim, Pant Aditya Bhushan
Primary Institution: Indian Institute of Toxicology Research, Lucknow, India
Hypothesis
Can dimetylthiourea (DMTU) and N-acetylcysteine (NAC) mitigate the cytogenotoxic effects of titanium dioxide nanoparticles and multi-walled carbon nanotubes in human lung cancer cells?
Conclusion
DMTU and NAC significantly reduce oxidative stress and genotoxicity markers in A549 cells exposed to titanium dioxide nanoparticles and multi-walled carbon nanotubes.
Supporting Evidence
- Both DMTU and NAC were effective in reducing ROS levels in A549 cells exposed to nanoparticles.
- Long-term treatment with DMTU and NAC showed better protection than short-term pretreatment.
- TiO2-NPs induced toxic responses were mediated through H2O2 generation.
- MWCNTs caused toxicity primarily by altering the enzymatic antioxidant system.
Takeaway
This study shows that two compounds, DMTU and NAC, can help protect lung cancer cells from damage caused by tiny particles found in some materials.
Methodology
The study involved exposing A549 cells to titanium dioxide nanoparticles and multi-walled carbon nanotubes, followed by treatment with DMTU and NAC to assess their protective effects.
Limitations
The study primarily focuses on in vitro conditions, which may not fully replicate in vivo responses.
Participant Demographics
Human lung cancer cells (A549) were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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