No Effect of Folic Acid Supplementation on Global DNA Methylation in Men and Women with Moderately Elevated Homocysteine
2011

Folic Acid Supplementation and DNA Methylation

Sample size: 216 publication 10 minutes Evidence: high

Author Information

Author(s): Jung Audrey Y., Smulders Yvo, Verhoef Petra, Kok Frans J., Blom Henk, Kok Robert M., Kampman Ellen, Durga Jane

Primary Institution: Radboud University Nijmegen Medical Center

Hypothesis

Does long-term folic acid supplementation increase global DNA methylation in individuals with elevated plasma homocysteine?

Conclusion

Long-term folic acid supplementation does not increase global DNA methylation in individuals with elevated homocysteine levels.

Supporting Evidence

  • Folic acid supplementation did not change global DNA methylation levels after three years.
  • Participants were matched on age and smoking status to control for these variables.
  • Significant increases in serum and erythrocyte folate were observed in the folic acid group.

Takeaway

Taking folic acid for a long time doesn't change how our DNA is marked, even if we have high levels of a certain chemical in our blood.

Methodology

Participants were randomly assigned to receive either folic acid or placebo for three years, and DNA methylation was measured in their blood.

Potential Biases

Potential bias due to the selection of participants based on specific health criteria.

Limitations

The study focused only on individuals with elevated homocysteine and did not explore effects in those with lower levels.

Participant Demographics

Dutch men and post-menopausal women aged 50-70 years.

Statistical Information

P-Value

0.79

Confidence Interval

95%CI = −0.05, 0.07

Statistical Significance

p=0.79

Digital Object Identifier (DOI)

10.1371/journal.pone.0024976

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