Yeast Growth on Plant Leaves Using Methanol
Author Information
Author(s): Kawaguchi Kosuke, Yurimoto Hiroya, Oku Masahide, Sakai Yasuyoshi
Primary Institution: Kyoto University
Hypothesis
How does the methylotrophic yeast Candida boidinii proliferate on the leaves of Arabidopsis thaliana in response to methanol dynamics?
Conclusion
The study found that Candida boidinii can proliferate on the leaves of Arabidopsis thaliana by utilizing methanol, which fluctuates in concentration throughout the day.
Supporting Evidence
- Candida boidinii proliferated 3 to 4 times on the leaves of Arabidopsis thaliana over 11 days.
- The local methanol concentration on the leaves varied significantly during the daily light-dark cycle.
- Yeast cells responded to methanol concentrations by altering gene expression related to methanol metabolism.
Takeaway
Yeast can live on plant leaves by eating methanol, which changes in amount during the day.
Methodology
The study used a fluorescent protein sensor to measure methanol concentrations and conducted qPCR to analyze yeast proliferation on plant leaves.
Limitations
The study primarily focused on one yeast species and one plant type, which may limit the generalizability of the findings.
Digital Object Identifier (DOI)
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