Enhanced Clathrin-Dependent Endocytosis in the Absence of Calnexin
2011
Calnexin Deficiency and Endocytosis
publication
Evidence: moderate
Author Information
Author(s): Li Hao-Dong, Liu Wen-Xin, Michalak Marek
Primary Institution: Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
Hypothesis
What is the role of calnexin in clathrin-dependent endocytosis?
Conclusion
Calnexin deficiency leads to increased clathrin-dependent endocytosis in neuronal cells.
Supporting Evidence
- Calnexin interacts with SGIP1, a protein involved in endocytosis.
- Calnexin-deficient cells show enhanced clathrin-dependent endocytosis.
- Expression of full-length calnexin reverses the increased endocytosis in deficient cells.
Takeaway
Calnexin helps control how cells take in materials, and without it, cells take in too much too quickly.
Methodology
The study used yeast two-hybrid screening and immunoprecipitation techniques to analyze protein interactions.
Digital Object Identifier (DOI)
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