Rac1 and Stathmin but Not EB1 Are Required for Invasion of Breast Cancer Cells in Response to IGF-I
2011
Rac1 and Stathmin Are Needed for Breast Cancer Cell Invasion
publication
Evidence: moderate
Author Information
Author(s): Shigeru Morimura, Kazuhide Takahashi
Primary Institution: Kanagawa Cancer Center Research Institute
Hypothesis
Are Rac1, stathmin, and EB1 necessary for the invasion of breast cancer cells in response to IGF-I?
Conclusion
Rac1 and stathmin are required for the invasion of MDA-MB-231 breast cancer cells in response to IGF-I, while EB1 is not.
Supporting Evidence
- Rac1 and stathmin were overexpressed in invasive MDA-MB-231 cells compared to noninvasive MCF7 cells.
- Depletion of Rac1 or stathmin significantly inhibited IGF-I-induced invasion of MDA-MB-231 cells.
- EB1 depletion did not affect the invasion of MDA-MB-231 cells.
Takeaway
Some proteins help cancer cells move and invade other areas. In this study, two proteins, Rac1 and stathmin, were found to be important for this process in a specific type of breast cancer cell.
Methodology
The study involved cell culture, lamellipodia formation assays, cell invasion assays, and RNA interference to assess the roles of Rac1, stathmin, and EB1.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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