Complexity of Bidirectional Transcription and Alternative Splicing at Human RCAN3 Locus
Author Information
Author(s): Facchin Federica, Vitale Lorenza, Bianconi Eva, Piva Francesco, Frabetti Flavia, Strippoli Pierluigi, Casadei Raffaella, Pelleri Maria Chiara, Piovesan Allison, Canaider Silvia
Primary Institution: Center for Research in Molecular Genetics (Fondazione CARISBO), University of Bologna, Bologna, Italy
Hypothesis
The study aims to explore the complexity of the human RCAN3 locus through in silico and in vitro analyses.
Conclusion
The study identifies 21 alternative spliced isoforms of the RCAN3 gene and a new antisense gene, RCAN3AS.
Supporting Evidence
- The study identified at least 21 alternative spliced isoforms of the RCAN3 gene.
- 16 of the isoforms were identified for the first time in this study.
- A new gene, RCAN3AS, was also identified, which overlaps with the RCAN3 gene.
Takeaway
Scientists found many different versions of a gene called RCAN3 in humans, which can help us understand how this gene works in our bodies.
Methodology
The study used RT-PCR and bioinformatic analyses to identify and analyze alternative splicing isoforms in human tissues.
Limitations
The study may not capture all possible isoforms due to the complexity of the RCAN3 locus and the limitations of the techniques used.
Participant Demographics
The study analyzed RNA from 17 different human tissues.
Digital Object Identifier (DOI)
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