Gene Expression in Medicago truncatula During Mycorrhizal Symbiosis
Author Information
Author(s): Gomez S Karen, Javot Hélène, Deewatthanawong Prasit, Torres-Jerez Ivone, Tang Yuhong, Blancaflor Elison B, Udvardi Michael K, Harrison Maria J
Primary Institution: Boyce Thompson Institute for Plant Research
Hypothesis
To identify genes involved in arbuscule development and function in Medicago truncatula during arbuscular mycorrhizal symbiosis.
Conclusion
The study identified novel genes expressed in Medicago truncatula roots during arbuscular mycorrhizal symbiosis, providing insights into the molecular events of this interaction.
Supporting Evidence
- 652 genes showed a 2-fold or greater change in gene expression in mycorrhizal roots relative to controls.
- 637 genes showed increased transcript levels in G. intraradices-colonized roots.
- New AM-induced genes include an ammonium transporter with a 4.8 fold induction in mycorrhizal roots.
- Five members of the GRAS transcription factor family were up-regulated in mycorrhizal roots.
- Transcripts for 10 G. intraradices genes were present in arbuscules.
Takeaway
This study looked at how certain genes work in plants when they team up with fungi to help each other grow. They found new genes that help this teamwork happen.
Methodology
The study used the Affymetrix GeneChip® Medicago Genome Array and laser microdissection to analyze gene expression in specific root cell types.
Limitations
The study's findings are based on a limited number of genes and may not represent the entire transcriptome involved in the symbiosis.
Statistical Information
P-Value
4.4E-154
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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