Fate of the H-NS–Repressed bgl Operon in Evolution of Escherichia coli
2009

Evolution of the bgl Operon in E. coli

Sample size: 174 publication 10 minutes Evidence: high

Author Information

Author(s): Sankar T. Sabari, Neelakanta Girish, Sangal Vartul, Plum Georg, Achtman Mark, Schnetz Karin

Primary Institution: Institute for Genetics, University of Cologne

Hypothesis

How has the bgl operon evolved in different phylogenetic groups of E. coli?

Conclusion

The bgl operon is maintained functionally in strains of the phylogenetic group B2, while it is eroded in groups A and B1, and absent in group D.

Supporting Evidence

  • The bgl operon is present in strains of phylogenetic groups A, B1, and B2.
  • 20% of strains in groups A and B1 have mutations in the bgl operon.
  • bgl is functional in almost all B2 isolates.

Takeaway

This study looks at a part of E. coli's DNA that helps it use certain sugars. Some E. coli can still use this part, while others have lost it over time.

Methodology

The study involved typing the bgl operon in 174 strains of E. coli and analyzing their phenotypes and phylogenetic relationships.

Potential Biases

Potential bias in strain selection and environmental conditions during analysis.

Limitations

The study may not account for all environmental factors affecting bgl operon expression.

Participant Demographics

The study included 171 E. coli isolates and 3 strains of Escherichia albertii.

Statistical Information

P-Value

0.00026

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pgen.1000405

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