MU2 and HP1a Regulate the Recognition of Double Strand Breaks in Drosophila melanogaster
2011
HP1a and MU2 in DNA Repair in Drosophila
publication
Evidence: moderate
Author Information
Author(s): Dronamraju Raghuvar, Mason James M.
Primary Institution: Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences
Hypothesis
What role does HP1 play in the formation of repair foci and cell cycle control in response to DNA damage?
Conclusion
HP1a interacts with MU2 and plays a significant role in the recognition and repair of DNA double strand breaks in Drosophila.
Supporting Evidence
- MU2 binds to HP1a in untreated cells, suggesting a direct interaction.
- HP1a depletion increases apoptosis and disrupts G2/M arrest in cells after irradiation.
- Repair foci formed in heterochromatin migrate to the periphery in an HP1-dependent manner.
Takeaway
This study shows that two proteins, HP1a and MU2, work together to help fix broken DNA in fruit flies.
Methodology
The study used yeast two-hybrid screens, co-immunoprecipitation, and immunocytochemistry to analyze protein interactions and DNA damage response.
Digital Object Identifier (DOI)
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