Two-point Kinetic Determination of Uric Acid in Serum
Author Information
Author(s): G. A. Harff, F. Lamie
Primary Institution: Department of Clinical Chemistry, Academic Hospital, Free University, Amsterdam, The Netherlands
Hypothesis
The new aldehyde dehydrogenase method can improve the determination of uric acid levels in serum using the KONE CD analyser.
Conclusion
The Boehringer Mannheim kit provides a quick and reproducible method for uric acid determination with good linearity.
Supporting Evidence
- The Boehringer Mannheim kit allows for 300 determinations per hour.
- The procedure provides good reproducibility for routine use.
- Only minor interference is expected from xanthine oxidase and xanthine.
Takeaway
This study shows a new way to measure uric acid in blood that is faster and more accurate than older methods.
Methodology
The study optimized a two-point kinetic method for uric acid measurement using the KONE CD analyser and compared it with existing methods.
Limitations
The Human kit could not be adapted for the two-point kinetic determination, and the KONE CD has a longer lag time affecting results.
Participant Demographics
Patients' sera from the Academic Hospital were used in correlation studies.
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