DnaC Inactivation in E. coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes
Author Information
Author(s): Løbner-Olesen Anders, Slominska-Wojewodzka Monika, Hansen Flemming G., Marinus Martin G.
Primary Institution: University of Massachusetts Medical School
Hypothesis
How does DnaC inactivation affect the SOS response and nucleotide biosynthesis gene expression in E. coli?
Conclusion
DnaC inactivation triggers the SOS response and increases the expression of nucleotide biosynthesis genes in E. coli.
Supporting Evidence
- DnaC inactivation leads to the induction of the SOS response in E. coli.
- Expression of nucleotide biosynthesis genes increases in response to DnaC inactivation.
- Flow cytometric analysis confirmed that dnaC2 cells had completed early stages of chromosome replication initiation.
- Induction of heat shock genes was observed in dnaC2 cells at non-permissive temperatures.
Takeaway
When a specific protein in E. coli is turned off, it causes the bacteria to react to stress and make more building blocks for DNA.
Methodology
DNA microarrays were used to measure mRNA levels in initiation-deficient mutants at different temperatures.
Limitations
The study did not identify cell cycle regulated genes in synchronized cells using microarray analysis.
Digital Object Identifier (DOI)
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