High-Cell Density Technique for Producing Hepatitis B Vaccine
Author Information
Author(s): Gurramkonda Chandrasekhar, Adnan Ahmad, Gäbel Thomas, Lünsdorf Heinrich, Ross Anton, Nemani Satish Kumar, Swaminathan Sathyamangalam, Khanna Navin, Rinas Ursula
Primary Institution: International Centre for Genetic Engineering & Biotechnology
Hypothesis
Can a simple fed-batch process enhance the production of Hepatitis B surface antigen using Pichia pastoris?
Conclusion
The study achieved a seven-fold increase in HBsAg production, making it more feasible to produce vaccines for resource-poor countries.
Supporting Evidence
- The process led to maximum concentrations of ~7 grams HBsAg per liter culture broth.
- More than 30% of the soluble protein was competent for assembly into virus-like particles.
- The study proposes a simpler method compared to existing multi-phase protocols.
Takeaway
Scientists found a better way to grow yeast that makes a part of the Hepatitis B vaccine, which could help more people get vaccinated.
Methodology
A two-stage fed-batch cultivation technique was used, starting with a glycerol batch phase followed by a constant methanol feeding phase.
Digital Object Identifier (DOI)
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