Genome-Scale Validation of Deep-Sequencing Libraries
Author Information
Author(s): Dominic Schmidt, Rory Stark, Michael D. Wilson, Gordon D. Brown, Duncan T. Odom
Primary Institution: Cancer Research UK, Cambridge Research Institute
Hypothesis
Can the Mab-seq method improve the quality evaluation of nucleic acid libraries for deep sequencing?
Conclusion
The Mab-seq protocol allows reliable detection of transcription factor binding sites and modified histones, providing quality-tested libraries for deep sequencing.
Supporting Evidence
- Mab-seq combines the ease of microarrays with the depth of high-throughput sequencing.
- The method allows for the reliable detection of transcription factor binding sites.
- Mab-seq provides a way to validate libraries before sequencing, saving time and resources.
Takeaway
This study shows a new method that helps scientists check if their DNA samples are good enough before doing expensive deep sequencing.
Methodology
The study developed a method called Mab-seq that uses microarrays to validate sequencing libraries before deep sequencing.
Limitations
The method may still be limited by the availability of high-throughput sequencing technology.
Digital Object Identifier (DOI)
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