Drosophila melanogaster PeptideAtlas: A Resource for Fly Proteomics
Author Information
Author(s): Loevenich Sandra N, Brunner Erich, King Nichole L, Deutsch Eric W, Stein Stephen E, The FlyBase Consortium, Aebersold Ruedi, Hafen Ernst
Primary Institution: Institute of Molecular Systems Biology, ETH Zurich
Conclusion
The Drosophila melanogaster PeptideAtlas provides a comprehensive resource of observed mass spectra matching to 76,724 fly peptides, confirming gene models and facilitating proteomics studies.
Supporting Evidence
- The PeptideAtlas confirms gene models for 9,263 protein isoforms.
- It provides evidence for the introduction of new transcripts.
- The database supports targeted proteomics experiments.
- PeptideAtlas facilitates improved genome annotation.
- Consensus mass spectra enhance peptide identification speed and accuracy.
Takeaway
The Drosophila PeptideAtlas is like a big library of tiny pieces of proteins that helps scientists understand more about fruit flies and their genes.
Methodology
The study involved analyzing protein extracts from Drosophila cells using liquid chromatography and tandem mass spectrometry to identify peptides.
Limitations
Some proteins corresponding to 35% of the gene models were not observed, and there are challenges related to the complexity of protein samples and potential errors in gene annotations.
Digital Object Identifier (DOI)
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