Gallus Heat shock cognate protein 70, a novel binding partner of Apoptin
2011
Gallus Heat Shock Cognate Protein 70 Binds to Apoptin
publication
Evidence: moderate
Author Information
Author(s): Chen Kun, Luo Zheng, Zheng Shijun
Primary Institution: China Agricultural University
Hypothesis
The study aims to identify Apoptin-associated proteins to understand the molecular mechanism of Apoptin's action.
Conclusion
Gallus Hsc70 is identified as an Apoptin binding protein that regulates RelA/p65 gene expression induced by Apoptin.
Supporting Evidence
- Hsc70 interacts with Apoptin in DF-1 cells.
- The binding domain of Apoptin with Hsc70 is located between amino acids 30-60.
- Hsc70 is translocated into the nuclei of DF-1 cells treated with Apoptin.
Takeaway
The study found that a protein called Hsc70 from chickens can stick to another protein called Apoptin, which helps understand how Apoptin works in cells.
Methodology
Yeast two-hybrid assays and co-immunoprecipitation were used to identify and confirm the interaction between Apoptin and Hsc70.
Digital Object Identifier (DOI)
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